Paper Title :Investigating The Multidrug Resistance Of Mrsa And The Genes Involved
Author :Soojung Lee, Youngchan Kim
Article Citation :Soojung Lee ,Youngchan Kim ,
(2015 ) " Investigating The Multidrug Resistance Of Mrsa And The Genes Involved " ,
International Journal of Advances in Science, Engineering and Technology(IJASEAT) ,
pp. 148-153,
Volume-3, Issue-4
Abstract : MRSA, which stands for Methicillin Resistant Staphylococcus Aureus, is a strain of the bacterium Staphylococcus
Aureus which is a coagulase positive gram positive bacterium. It is very dangerous, resistant to drugs, and can even cause
serious, life-threatening problems. This study is focused on identifying the gene that is related to drug resistance. For the
investigation of the multidrug resistance of MRSA, a sample of MRSA was obtained from a local hospital. The DNA from
MRSA was extracted and mixed with E. coli for transformation of the host bacteria. The resistance of the transformed E. coli
was tested in different concentrations of ampicillin, gentamycin, kanamycin, and tetracycline. To investigate the genes that
were involved in the multidrug resistance, I first prepared the transformed E. coli via mini prep, and then ran the E. coli through
PCR. Finally, the method of gel electrophoresis was used to identify the genes involved in drug resistance. According to the
results, both MRSA and the genetically transformed E. coli show drug resistance to some antibiotics. It can be interpreted that
the DNA that codes for antibiotic resistance was successfully transported into the E. coli that survived the antibiotics. The
DNA was amplified in both the drug resistant MRSA and E. coli through PCR. Since the section of DNA that codes for the
multidrug resistance is most likely shared in all the bacterium that survived the antibiotics, the DNA involved in drug
resistance can be identified through electrophoresis.
Index Terms- ampicillin, gentamycin, kanamycin, MRSA (Methicillin Resistant Staphylococcus Aureus), multidrug
resistance, tetracycline
Type : Research paper
Published : Volume-3, Issue-4
DOIONLINE NO - IJASEAT-IRAJ-DOIONLINE-3058
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Copyright: © Institute of Research and Journals
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Published on 2015-10-10 |
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