Paper Title :Development And Validation Of Ondansetron Hydrochloride In Pharmaceutical Dosage Form By Rp-Hplc Method
Author :T. B. Deshmukh, s. S. Deo, F. Inam, T. L. Lambat, S. B. Gurubaxani, A. V. Choudhari
Article Citation :T. B. Deshmukh ,s. S. Deo ,F. Inam ,T. L. Lambat ,S. B. Gurubaxani ,A. V. Choudhari ,
(2015 ) " Development And Validation Of Ondansetron Hydrochloride In Pharmaceutical Dosage Form By Rp-Hplc Method " ,
International Journal of Advances in Science, Engineering and Technology(IJASEAT) ,
pp. 15-20,
Special Issue-1
Abstract : A simple, rapid, economical, accurate, and precise
reverse phase high performance liquid
chromatography (RP-HPLC) method was developed
and subsequently validated for the estimation of
Ondansetron hydrochloride (ODH) in bulk and
pharmaceutical dosage form. The analysis was
carried out using Princeton SPHER C18 column (250
mm x 4.6 mm id, 5µ particle size) at 300
c temperature
using mobile phase Buffer:Acetonitrile:Methanol
(50:40:10% v/v/v) (pH 4.5 ± 0.05) at flow rate 1.0
ml/min. Quantification was achieved with UV
detector at 248 nm. Retention time of Ondansetron
hydrochloride was found to be 2.67 ± 0.05 min.
Linearity was studied in the concentration range 10-
60 µg/ml for Ondansetron hydrochloride with a
correlation coefficient of 0.9982. The proposed
method was validated according to ICH guidelines
with respect to linearity, accuracy, precision,
robustness, LOD, and LOQ. The developed method
with good symmetry, successfully applied for
determination of Ondansetron hydrochloride in its
pharmaceutical dosage form.
Keywords: RP-HPLC, Ondansetron hydrochloride,
Validation.
Type : Research paper
Published : Special Issue-1
DOIONLINE NO - IJASEAT-IRAJ-DOIONLINE-2419
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Copyright: © Institute of Research and Journals
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Published on 2015-06-19 |
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