Paper Title :Rapid Antibiotic Susceptibility Testing using a Micro-Engineered Sensor and Electronic Platform

Author :Anil Vishnu G K, Nitu Bhaskar, Arjun B. S., Misaal Khan, Prathik B. H.,Hardik J. Pandya

Article Citation :Anil Vishnu G K ,Nitu Bhaskar ,Arjun B. S. ,Misaal Khan ,Prathik B. H. ,Hardik J. Pandya , (2022 ) " Rapid Antibiotic Susceptibility Testing using a Micro-Engineered Sensor and Electronic Platform " , International Journal of Advances in Science, Engineering and Technology(IJASEAT) , pp. 102-105, Volume-10,Issue-1

Abstract : Abstract - The emergence and spread of antibiotic resistance is a global healthcare concern. Incorrect prescription of antibiotics and the indiscriminate use of broad-spectrum antibiotics is a major cause of antibiotic resistance-related mortality worldwide. Conventional techniques used to determine the minimum inhibitory concentration (MIC) of a drug against the bacteria are time-consuming (24-48 h) and prone to false-negative results. There is an urgent need to develop a point-of-care antibiotic susceptibility testing (AST) platform for detecting pathogens and rapidly (<120 minutes) identifying the efficacy of the antibiotics. Here, we report a microchip consisting of interdigitated electrodes inside a microwell for rapid, real-time, and label-free antibiotic susceptibility testing based on electrical sensing modality. A portable impedance analyzer with a port for holding the microchip for AST testing is also developed. We have used E. coli as a clinical model with ampicillin antibiotic to evaluate the performance of the microchip. The results from the microchips are compared with the conventional antibiogram assays and bacteria viability tests. Keywords – Micro fabrication, Antibiotic Resistance, Antibiotic Susceptibility Testing, Point-of-Care Platforms.

Type : Research paper

Published : Volume-10,Issue-1

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