Paper Title :Two Organophosphorous Amino Acid Analogues Enhance Sensitivity of Some Plant Pathogenic Fungi to the Folicur Fungicide
Author :Vitaly G. Dzhavakhiya, Tatiana M. Voinova, Natalia V. Statsyuk, Larisa A. Shcherbakova
Article Citation :Vitaly G. Dzhavakhiya ,Tatiana M. Voinova ,Natalia V. Statsyuk ,Larisa A. Shcherbakova ,
(2018 ) " Two Organophosphorous Amino Acid Analogues Enhance Sensitivity of Some Plant Pathogenic Fungi to the Folicur Fungicide " ,
International Journal of Advances in Science, Engineering and Technology(IJASEAT) ,
pp. 16-19,
Volume-6, Issue-4
Abstract : The ability of two organophosphorous analogues of natural amino acids, 1-(aminoethyl)phosphinic acid (AEPA)
and (aminomethyl)thiophosphonic acid (AMTPA), able to inhibit the biosynthesis of fungal polyketide mycotoxins, to
enhance the antifungal action of the Folicur commercial fungicide (a.i. tebuconasole) on some plant pathogenic fungi
(Phoma glomerata, Fusarium culmorum, and Alternaria alternata) has been evaluated. The tested compounds and Folicur
were added to PDA or minimal medium in sub-fungicidal concentrations providing no or slight suppression of a fungal
growth. The fungal growth suppression level was determined by a colony diameter measurement. A possible synergism
between Folicur and AEPA or AMTPA resulting in a sensitization of fungi to the fungicide was determined using a Limpel’s
criterion: the observed growth-inhibitory effect (Er) of a fungicide co-applied with a putative sensitizer should exceeded the
expected additive effect of the treatment with these two substances (Ee) calculated by the Limpel’s formula. In the case of
co-application of AEPA (0.01%) with Folicur (0.25 ppm) on PDA medium, Er exceeded Ee for all three fungi (55% vs.
38.94% for P. glomerata, 44% vs. 28% for A. alternata, and 63% vs. 57% for F. culmorum) that confirmed the existing
synergistic effect between these compounds. To exclude possible competition between amino acids containing in the
medium and their phosphoanalogues, a new experiment was arranged for F. culmorum using minimal medium. The
synergistic character of the AEPA co-application with Folicur (0.01 ppm) was confirmed for two concentrations of this
compound, 0.0001 and 0.0002%; in the first case, Er (15.9%) > Ee (10.22%), and in the second case, Er (22.4%) > Ee
(10.07%). Another putative sensitizer, AMTPA, combined with Folicur (0.01 ppm), also confirmed its synergetic action (Er
(29.0%) > Ee (20.83%)). The obtained results showed some prospects in the search for inexpensive commercially available
compounds possessing a sensitizing effect toward plant pathogenic fungi, which may be used as a low-cost solution for
reduction of effective dosages of commercial fungicides to prevent pesticide contamination of the environment.
Keywords: Chemosensitization, plant pathogenic fungi, fungicide, polyketide biosynthetic pathway, Folicur,
organophosphorous analogues of amino acids
Type : Research paper
Published : Volume-6, Issue-4
DOIONLINE NO - IJASEAT-IRAJ-DOIONLINE-13899
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Copyright: © Institute of Research and Journals
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Published on 2018-12-25 |
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