Paper Title :Direct Rhizogenesis, Phytochemical Analysis and Antimicrobial Activity of Bryonia Laciniosa
Author :Ujjwala Supe
Article Citation :Ujjwala Supe ,
(2018 ) " Direct Rhizogenesis, Phytochemical Analysis and Antimicrobial Activity of Bryonia Laciniosa " ,
International Journal of Advances in Science, Engineering and Technology(IJASEAT) ,
pp. 18-20,
Volume-6, Issue-1
Abstract : Efficient rhizogensis was established for Bryonia laciniosa using leaf derived calli. The maximum morphogenic
callus induction rate (80%) was observed from leaf explant by culturing in MS medium supplemented with 2.0 mg/l IAA +
2.0mg/l IBA. Calli size and fresh weight increased substancially through subculturing. The highest percentage of root
induction (70%) and highest mean number of roots (12.33) per culture were obtained with 2.0 mg/l IAA + 2.0 mg/l IBA.
Phytochemicals like alkaloids, tannins, flavonoids, saponins and glycosides were also found present. The study indicates the
presence of nutritional and chemical components that are beneficial in addition to the numerous medicinal values of the
plant. Antibacterial effect of Bryonia laciniosa was evaluated on bacterial strains like Bacillus cereus, Staphylococcus
aureus, Pesudomonas Enterobacter aerogenes, Escherichia coli and Klebsiella pneumoniae. The solvents used for the
extraction of plants were water and methanol. The in vitro antibacterial activity was performed by agar disc diffusion. The
most susceptible bacteria was K. pneumoniae. The extracts of callus and plant material could not inhibit any of the bacterial
strains investigated.
Keywords - Rhizogenesis, Antimicrobial, Bryonia Laciniosa, Phytochemical Analysis
Type : Research paper
Published : Volume-6, Issue-1
DOIONLINE NO - IJASEAT-IRAJ-DOIONLINE-10845
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Published on 2018-03-30 |
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