
<strong>Paper Title</strong><br>
Study on Optimization of Lipase: Potential Application in Detergent Industries<br>
<br>

<strong>Abstract</strong><br>
Synthesis of biodegradable products for industrial applications have emerged with considerable biotechnological
significance. Thus, an approach to search the lipase in detergent industry with improved production is characterized by an
isolation of a solvent tolerant bacteria from the local environment with high activity in alkaline pH and, stability in the
presence of organic solvents. We have tried in making it cost efficient and commercially important enzyme by media
optimization. Maximum lipase production was obtained at pH 9 and temperature at 37°C. Purification of lipase was carried
out with ammonium sulphate precipitation and gel filtration chromatography. SDS-PAGE estimated the molecular mass of
lipase protein to be 27kDa.
Keywords- Lipase; Solvent tolerant; Alkaline pH; Detergents