Paper Title :Isolation of Leptospira in Suspected Cases of Leptospirosis: Is Heparinized Whole Blood a Better Modality
Author :Ashvika Sarwankar, Dr. Summaiya Mulla
Article Citation :Ashvika Sarwankar ,Dr. Summaiya Mulla ,
(2016 ) " Isolation of Leptospira in Suspected Cases of Leptospirosis: Is Heparinized Whole Blood a Better Modality " ,
International Journal of Advances in Science, Engineering and Technology(IJASEAT) ,
pp. 150-152,
Volume-4,Issue-4, Spl. Iss-2
Abstract : Leptospirosis is caused by pathogenic bacteria called leptospires that are transmitted directly or indirectly from
animals to humans. It occurs worldwide but is most common in tropical and subtropical areas. It is a potentially serious but
treatable disease. Its symptoms may mimic those of a number of other unrelated infections such as influenza, meningitis,
hepatitis, dengue, or other viral hemorrhagic fevers. Patients residing in the peripheral areas of South Gujarat may not have
easy accessibility to the health care facilities. Also the culture sample if taken in the early course of infection can show good
isolation rates. Considering these aspects, this study was planned with the objective to note the isolation rates using
heparinized whole blood and compare them with direct culture into the culture media. A total of 221 samples were taken,
culture being done by both the methods as mentioned above. Average isolation rate from all the samples was 7.2%. Out of
them isolation rate for heparinized whole blood sample was 100% and that for direct culture was 62.5% only. Also the
contamination rate with heparinized whole blood was 23.9% and for direct blood culture it was 71.9%. The maximum
duration for which the heparinized blood was stored before inoculating them in the culture media and that showed growth
was about 8 days to 56 days. So it can be said that heparinized whole blood for isolation of Leptospira is the best modality,
as it has low contamination rate, more isolation rate and the time period before which the heparinized sample has to be
inoculated in the culture media is more.
Type : Research paper
Published : Volume-4,Issue-4, Spl. Iss-2
DOIONLINE NO - IJASEAT-IRAJ-DOIONLINE-6631
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Published on 2017-02-03 |
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