Paper Title :A Bi-Functional Xylanase/ Xyloglucanase, GH5H, From Termite Metagenome Improves the Hydrolysis of Pretreated Biomass
Author :Abhishek Bhattacharya, Mpho Mafa, Konanani Rashamuse, Brett I. Pletschke
Article Citation :Abhishek Bhattacharya ,Mpho Mafa ,Konanani Rashamuse ,Brett I. Pletschke ,
(2016 ) " A Bi-Functional Xylanase/ Xyloglucanase, GH5H, From Termite Metagenome Improves the Hydrolysis of Pretreated Biomass " ,
International Journal of Advances in Science, Engineering and Technology(IJASEAT) ,
pp. 213-216,
Volume-4,Issue-3, Spl. Iss-1
Abstract : Xyloglucan is a major component of the primary cell wall of higher plants and forms an intimate network with
cellulose through hydrogen bonding and also with hemicellulosic components. In the present study, a bi-functional
xylanase/xyloglucanase (GH5H) from a termite metagenome with high xylanase and xyloglucanase activities was used to
replace Aspergillus niger xylanase in a previously established enzyme cocktail. The new combination was found to increase
both glucose and xylose release from pre-treated biomass. Substrate competition assay indicated that xylanase and
xyloglucanase activity is catalysed by the same active center.
Keywords— Bi-functional enzyme, lignocellulose hydrolysis, termite GH5H.
Type : Research paper
Published : Volume-4,Issue-3, Spl. Iss-1
Copyright: © Institute of Research and Journals
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Published on 2016-09-12 |
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