International Journal of Advances in Science, Engineering and Technology(IJASEAT)
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Volume-7,Issue-3  ( Jul, 2019 )
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Sep
Submitted Papers : 80
Accepted Papers : 10
Rejected Papers : 70
Acc. Perc : 12%
  Journal Paper

Paper Title
Development And Validation Of UPLC Methods For Simultaneous Determination Of Selected Antihypertensive Drugs In Their Dosage Forms

Abstract
Accurate, precise and sensitive UPLC methods were developed and validated for the simultaneous determination of selected antihypertensive drugs; a binary mixture containing Lercanidipine HCl (LER) and Enalapril maleate (ENA), and a ternary mixture containing amlodipine besylate (AML), olmesartan medoxomil (OLM) and hydrochlorothiazide (HCT). Chromatographic separation was achieved using Kinetex™ column – C18 (4.6 x 150 mm, 2.6 μm). Isocratic elution at a flow rate 1 mL/min was performed using a mobile phase of methanol: phosphate buffer pH = 3.0 ± 0.1 (65: 35 v/v), for the binary mixture and acetonitrile: phosphate buffer pH = 3.0 ± 0.1 (45: 55 v/v), for the ternary mixture. The methods developed were satisfactorily applied to the analysis of the pharmaceutical formulations and proved to be specific and accurate for the quality control of the cited drugs in pharmaceutical dosage forms. The obtained results are statistically compared with those obtained by the official or reported methods, showing no significant difference with respect to accuracy and precision at p=0.05. Index Terms- antihypertensive, binary mixture, ternary mixture, UPLC.


Author - Hanan A. Merey, Nesrin K. Ramadan, Sherine S. Diab, Azza A. Moustafa

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