Paper Title :Analytical Method Development And Validaton Of RP HPLC For Simultaneously Estimation Of Chlorpheniramine Maleate, Dextromethorphen Hydrobromide, Phenylephrine Hydrochloride In Cough Syrup
Author :Kapildev R. Jaiswal
Article Citation :Kapildev R. Jaiswal ,
(2016 ) " Analytical Method Development And Validaton Of RP HPLC For Simultaneously Estimation Of Chlorpheniramine Maleate, Dextromethorphen Hydrobromide, Phenylephrine Hydrochloride In Cough Syrup " ,
International Journal of Advances in Science, Engineering and Technology(IJASEAT) ,
pp. 35-39,
Volume-4,Issue-1
Abstract : It is simple, accurate, precise, rapid, selective and reproducible reverse phase high performance liquid
chromatographic (RP-HPLC) method for simultaneous estimation of Dextromethorphen Hydrobromide, Phenylephrine
Hydrochloride of Chlorpheniramine Maleate in Cough syrup. The first method of three drugs which involves absorbance
measurement at 265 nm. Linearity was obtained in the range of 5-15 μg/ml, 2.5-7.5 μg/ml and 1-3 μg/ml respectively. Good
Chromatographic separation was achieved isocratically at 35 o C ± 0.5o C on Partisil 10 SCX column (250 × 4.6 mm i.d.)
with a mobile phase composed of Buffer: Methanol the ratio of 40:60 % V/V at flow rate of 1.0ml/min. The retention time of
Phenylephrine Hydrochloride, Dextromethorphen Hydrobromide, and Chlorpheniramine Maleate retention time was found
to be 5.0 min, 8.0 min., and 13.0min respectively. The correlation coefficient for calibration curve of all three peaks was
found to be 0.9999. The developed methods were validated according to ICH guidelines.
Keywords- Dextromethorphen Hydrobromide, Phenylephrine Hydrochloride of Chlorpheniramine Maleate, HPLC,
Validation.
Type : Research paper
Published : Volume-4,Issue-1
DOIONLINE NO - IJASEAT-IRAJ-DOIONLINE-3637
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Published on 2016-01-07 |
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