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Paper Title :
In Vitro Behavior of Zn-Modified Calcium Phosphate Fine Powders

Author :Boyka Andonova-Lilova, Radostina Alexandrova, Kostadinka Sezanova, Rumiana Gergulova, Diana Rabadjieva

Article Citation :Boyka Andonova-Lilova ,Radostina Alexandrova ,Kostadinka Sezanova ,Rumiana Gergulova ,Diana Rabadjieva , (2019 ) " In Vitro Behavior of Zn-Modified Calcium Phosphate Fine Powders " , International Journal of Advances in Science, Engineering and Technology(IJASEAT) , pp. 1-6, Volume-7,Issue-1

Abstract : Zinc modified (Zn/(Zn+Ca) = 0, 0.03, 0.13) calcium phosphate fine powders were synthesized by biomimetic approach and subsequent high-temperature treatment. Bi-phase mixture of β-tricalcium phosphate (β-TCP) and hydroxyapatite (HA) was prepared at non modified sample and monophase Zn-β-TCP at Zn modified samples. The in vitro biological behavior of the prepared powders was studied using human Lep-3 and bovine MDBK cells. The effect of the materials on cell viability and proliferation was evaluated by three methods with different molecular / cellular targets and mechanisms of action - MTT test, neutral red uptake assay and crystal violet staining. The results obtained revealed high viability of both Lep-3 and MDBK cells cultured for 72h and 96h in the presence of the materials examined applied as 100% material-modified cell culture medium in all three cases. The dilution of material-modified cell culture medium with nonmodified culture medium leads to increasing of cell viability.

Type : Research paper

Published : Volume-7,Issue-1


DOIONLINE NO - IJASEAT-IRAJ-DOIONLINE-14944   View Here

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