Paper Title :Antioxidant Activity Fraction of Kaffir Lime Leaves (Citrus Hystrix Dc.) By 1,1-Diphenyl-2-Picrylhydrazil (DPPH) Radical Scavenging Method
Author :Nur Chaerun Nisa, Aktsar Roskiana Ahmad, Rezki Amriati Syarif, Andi Amaliah Dahlia
Article Citation :Nur Chaerun Nisa ,Aktsar Roskiana Ahmad ,Rezki Amriati Syarif ,Andi Amaliah Dahlia ,
(2018 ) " Antioxidant Activity Fraction of Kaffir Lime Leaves (Citrus Hystrix Dc.) By 1,1-Diphenyl-2-Picrylhydrazil (DPPH) Radical Scavenging Method " ,
International Journal of Advances in Science, Engineering and Technology(IJASEAT) ,
pp. 11-15,
Volume-6, Issue-3
Abstract : The sample used in this study is the leaves of Citrus hystrix DC. belong to the family Rutaceae. This research
aimed to determine the antioxidant activity of Citrus hystrix DC. leave fraction using 1,1-diphenyl-2-picrylhydrazil (DPPH)
free radical scavenging method. Citrus hystrix DC. leaf extract was extracted by maceration using ethanol 96% and obtained
ethanol extract yield value of 5,044% and subsequently fractionated by Vacuum Liquid Chromatography (VLC) with the
mobile phase is n-hexane:ethyl acetate and ethyl acetate:methanol. The stationary phase used silica gel F254. The number of
fractions obtained are 4 fractions. The results showed that the Citrus hystrix DC. leave fraction has antioxidant activity. The
value of IC50 fraction 1 is 275,814 μg/mL, fraction 2 is 200,297 μg/mL, fraction 3 is 242,247 μg/mL and fraction 4 is
121,831 μg/mL. The 4thfraction of the Citrus hystrix DC. leave has higher antioxidant than the other fractions.
Index terms - Antioxidant, Citrus hystrix DC., kaffir lime leaves, 1,1-diphenyl-2-picrylhydrazil (DPPH), Vacuum Liquid
Chromatography (VLC).
Type : Research paper
Published : Volume-6, Issue-3
Copyright: © Institute of Research and Journals
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Published on 2018-09-07 |
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