International Journal of Advances in Science, Engineering and Technology(IJASEAT)
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  Journal Paper

Paper Title
Isolation, Characterization and Identification of DNA’S Lactic Acid Bacteria (LAB) of Dadih Lintau (Tanah Datar District) which is Antibiotic Resistance

Abstract
Research about isolation, characterization, and identification of DNA’s lactic acid bacteria of dadih Lintau Tanah Datar District which is antibiotic resistant has been done. Eleven isolates were isolated; U4, U5, U6, U7, U8, U9, U10, U11, U12, U13 and U14; characteristic of isolates are broken white, round shape, convex, edge slick and Gram positive bacteria. Nine (9) isolates are rod cell, 2 others are cocci. isolates were characterized based on antibiotic resistance. Ampicillin 10 μg (AM 10), Diclixacillin 1 μg (DCX1), Gentamicin 10 μg (CN 10), Cofeperazone 75μg (CFP 75), Carbenicillin 100μg (CB 100) have been used as antibiotics test. The result showed that six isolates resistance to all of antibiotics; U4, U5, U6, U7, U8 and U11. The result of DNA identification by primer 27F-1429R, showed that PCR product about 1500 base pair. BLAST analisys result from all dadih Lintau isolate include the lactic acid bacteria group, match 97% - 100% with Lactobacillus plantarum. Keyword - Antibiotic, Buffalo Milk, Dadih, Fermented, Lactic Acid Bacteria


Author - Endang Purwati, James Hellyward, Yuherman, Hendri Purwanto, Puji Hartini R

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| Published on 2018-06-29
   
   
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