Paper Title :Β-Glucosidase Enzyme Screening from Shoot of Tabebuia Argentea

Author :Chariwat Pitsanuwong, Juthamanee Boonwan

Article Citation :Chariwat Pitsanuwong ,Juthamanee Boonwan , (2018 ) " Β-Glucosidase Enzyme Screening from Shoot of Tabebuia Argentea " , International Journal of Advances in Science, Engineering and Technology(IJASEAT) , pp. 37-39, Volume-6, Issue-2

Abstract : This work aims to purify the novel β–glucosidase enzyme from shoot of Tabebuia argentea. The enzyme extracted by using acetate buffer pH 5.5 from the fresh sample. The fractionation using for preliminary purification by ammonium sulfate ((NH4)2SO4) salt precipitation in the different salt concentration (0-30%, 30-60%, and >60%). The enzyme activity was measured under basic codition by using hydrolysis reaction of glycosidic bond by using p-nitrophenyl-D-glucopyranoside (pNPG) as an enzyme substrate. The λmax of corresponding p-nitrophenolate product under basic condition was detected at 405 nm. The enzyme activity via pNPG hydrolysis of seeds extract responds toward around 10-fold over the other part extracts of Tabebuia argentea, follow by selected enzyme fraction of shoot extract to subject to further chromatographic purification and enzymatic properties test before apply to be a biocatalyst in biological process. Index Terms - β–glucosidase, shoot of Tabebuia aurea, p-nitrophenyl-D-glucopyranoside

Type : Research paper

Published : Volume-6, Issue-2

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