Paper Title
Species Conservation Through Applied Seed Research With Special Reference to Careya Arborea (Kumbhi): A High Value Lesser Known Medicinal Tree Species of Central India

Careya arborea is a deciduous, small to medium-sized tree growing up to 20 meters tall. It belongs to family Myrtaceae and is a significant medicinal plant known as Kumbhi in Ayurveda. Though, most of the plant parts are used in traditional systems of medicine, bark and leaves constitute the important medicinal parts. The review reveals that numerous phytochemical constituents have been isolated from the plant possessing hepatoprotective, antioxidant, antimicrobial, anticoagulant, analgesic, antidiarrhoeal and various other important activities. Leaves are used in filaria, colic, loose motions and ulcers. Bark is used as an antipyretic, abortifacient, antipruritic and in smallpox, urinary discharges and rheumatic pain. Since last few decades, extensive exploration has been done to establish the biological activities and pharmacology of the extracts and plentiful chemical constituents including flavonoids, tannins, alkaloids, terpenoids and many other have been isolated. The regeneration and establishment of the plant is very slow and they are suppressed by weeds. The seed longevity is very low under short lived behaviour. So the growing concern in commercialization of plant medicines has led to over exploitation of this plant. In this connection ex-situ conservation through seeds and further development of packages of seed techniques for protecting these dwindling populations. The present study was undertaken to standardize the methods of collection, storage, pretreatments of seeds Careya arborea (Roxb.). This species was problematic species with reference to the storage and germination potential. Different pretreatments were applied with fresh and stored seeds at room temperature and tested for germination characteristics at 15 days intervals. The effects of different pretreatment on germination percent, germination velocity index, length of seedlings in both fresh and stored seeds were observed. The data reveals that in control the germination of fresh seeds was found to be 86%. However, stored seeds after 15, 30 and 45 days gave germination as 12%, 3% and 0% respectively. Among different pretreatments tried, the maximum germination 98% was recorded with seed soaking in 500 ppm IBA for 10 minutes in fresh seeds. However, stored seeds treated with IBA (500ppm for 10 minutes) gave 26%, 9% and 0% germination after 15, 30 and 45 days stored seeds respectively. Similarly germination velocity index and seedling growth was also found to be promising owing to promoted root and shoot length of seedling with seeds treated with 500 ppm IBA for 10 minutes. Besides this the fruit stored at 4°C temperature and were depulped after 15, 30 and 45 days and seeds taken for testing of germination potential. The data reveals that, the highest germination 88%, 62%, 26% and 6% was found with seed soaking in 500ppm IBA for 10 minutes after 15, 30, 45 and 60 days of storage at 4°C temperature against 71%, 37%, 18% and 4% in control respectively. Keywords— Pretreatments, Germination Velocity Index (GVI), Seedling growth, Germination potential, IBA. Exploration, Alkaloids.