Study on Effect of Horseradish Peroidase for the Control of Hydrogen Peroxide Mediated Singal Generation through the Growth of Gold Nanoparticles
In classical colorimetric immunoassays the signal for detection is generated by a bio-catalytic conversion of a
molecule (antigen or antibody) by an enzyme which acts as an amplifier. However, a sensitive and reliable immunoassay
requires high selectivity and affinity of antibodies to their corresponding antigens that forms the principle of bio-recognition.
If the presence of analyte is at very low levels, immunoassay is unable to detect them and as such necessitates the use of
more sensitive methods. Nanoparticles (e.g. gold or silver colloids etc) represent a new signal generation mechanism for biosensing
giving the required improvements in signal production. A catalytic approach involving hydrogen peroxide and gold
nanoparticles has recently been demonstrated and has the potential to be applied to a wide variety of food safety targets such
as chemical and microbiological contaminants, veterinary drug residues, and protein biomarkers.
Keywords - Immunoassay, Colorimetric, Bio-Recognition, Nanoparticle, Plasmonic, Contaminant.