<strong>Paper Title</strong><br>
Total Phenolic, Flavonoid Contents, Reducing Power and Free Radical Scavenging Potential of Pleurospermum Brunonis<br>
<br>

<strong>Abstract</strong><br>
In the present investigation it was observed that the chloroform extract of Pleurospermum brunonis  (PCH) 
possess high total phenolic content (24.03±0.71) whereas maximum total flavonoid content (3.39±0.15) was observed in 
butanol extract (PBU). The reducing potential of butanol extracts was better than ascorbic acid. The DPPH scavenging 
activity at 400 (93.73±1.11) and 500 µg/ml (96.26±0.93) is almost similar to standard in butanol extract. Antioxidants are 
substances that help prevent certain types of cell damage, especially those caused by oxidation. When certain types of 
oxygen molecules are allowed to travel freely in the body they cause what is known as oxidative damage which leads to the 
formation of free radicals. Free radicals are very dangerous to the body’s tissues and have been connected to cancer and 
premature aging.   
Keywords— Pleurospermum brunonis, free radicals, antioxidant assays, Phenolic compounds, Flavonoid.