Paper Title
Study on Optimization of Lipase: Potential Application in Detergent Industries

Synthesis of biodegradable products for industrial applications have emerged with considerable biotechnological significance. Thus, an approach to search the lipase in detergent industry with improved production is characterized by an isolation of a solvent tolerant bacteria from the local environment with high activity in alkaline pH and, stability in the presence of organic solvents. We have tried in making it cost efficient and commercially important enzyme by media optimization. Maximum lipase production was obtained at pH 9 and temperature at 37°C. Purification of lipase was carried out with ammonium sulphate precipitation and gel filtration chromatography. SDS-PAGE estimated the molecular mass of lipase protein to be 27kDa. Keywords- Lipase; Solvent tolerant; Alkaline pH; Detergents