One-Step DNA Extraction For Detection Of Listeria Monocytogenes
Conventional routine detection of Listeria monocytogenes in food products requires at least 7 days. Thus, the
rapid technology for detection of L. monocytogenes is of the utmost importance and urgent necessity. This study aims to
develop the rapid DNA extraction for detection of L. monocytogenes by using PCR assay. After DNA extraction of L.
monocytogenes, three methods including the deionized nanopure water and 0.001% SDS solution with or without boil
methods cloud be used for the one-step DNA extraction.
Keywords- one-step DNA extraction, Listeria monocytogenes, polymerase chain reaction.