Investigation Of The Apoptotic Pathway Induced By C3-Pyrazole/Chalcone Linked Beta-Carboline Hybrids Against Human Breast Cancer Cells Mcf-7
Cancer is a serious disease with a complex pathogenesis, which threats human life greatly. In our previous
studies C3-Pyrazole/Chalcone Linked Beta-Carboline Hybrids were synthesized and evaluated for their cytotoxic activity in
a panel of five human cancer cell lines and the new molecules like 7d and 8d were considered as potential leads. These
observations prompted us to investigate the apoptotic mechanism underplaying the cytotoxic activity of these compounds in
human breast cancer cells MCF-7. In the present investigation studies like flowcytometry of mitochondrial membrane
potential (ΔΨm), generation of reactive oxygen species (ROS) and Annexin V-FITC assay revealed that these compounds
induced mitochondrial mediated apoptosis in human breast cancer cells (MCF-7). It was further confirmed by western blot
analysis of pro apoptotic protein Bax and anti apoptotic protein Bcl-2.
Keywords— Anticancer activity, Flowcytometry, Western blot analysis, Mitochondrial mediated apoptosis.