Factor Predicting Total Nucleated Cell Count In Cord Blood Units Collected At King Abdullah International Medical Research Center Cord Blood Bank
Stem cells originating from neonatal cord blood have been used worldwide in transplant medicine to
treat various diseases. Stem cells efficacy in the umbilical cord blood (UCB) can be predicted by the number of
total nucleated cells (TNC). To optimize the clinical use of stem cell in our population, this study addresses
several variables affecting TNC count.
This is an observational cross- sectional study conducted at King Abdulaziz Medical City. From 2012 to 2014,
957 UCB units were collected from consented mothers by trained personnel using standard procedure. Data
analysis of clinically accepted CBUs was correlated with maternal and infant factors. The demographic data of
our subjects included maternal age(means ± SE: 29±0.18) and gestational age( 39±0.04). The [Median (IQR)] of
the mother’s gravidity [3(4)], parity [2(3)], abortion [0(1)] and the number of living children [2(3)]. Based on the
TNC accepted level of banking which is at least 90x107 cells, 188 CBUs (19.64 %) were rejected from a total of
957 units. Of the 17 maternal and infant variables evaluated, three factors demonstrated a statistically significant
predictive value of accepted TNC level. Cord blood volume was the best predictive factor (p-value=<0.0001).
Consequently, newborn birth weight (p-value =0.025) and method of delivery (P-value= 0.002).
Several maternal, neonatal and obstetric factors appear to play a major role in predicating accepted TNC count
level which can be used to improve the criteria of the donation of stem cells in cord blood unit.