Determination Of Selected Pesticide Residues In Fruits Using Quechers Approach And Reversed-Phase High Performance Liquid Chromatography (RP-HPLC)
This paper presents the application of reverse phase high performance liquid chromatographic (RP-HPLC) method
equipped with UV detector for the determination of five pesticides, in common fruit samples. The reverse phase liquid
Chromatography were used for the monitoring of pesticide residues employing QUEChERS approach of selected
organophosphate pesticide Monocrotophos, 2.4 D, Chlorpyrifos, Malaxon, Isopruton, and Phorate sulphoxide. Proposed
method was successfully applied for the determination of pesticide residues in orange, apple, pomegranate and grape fruits.
Average recoveries achieved for all the pesticides at fortification levels of 1, 0.5, 0.1 mg/l in analyzed fruits were above 90%
with relative standard deviations (RSD) less than 6%. The correlation coefficients (r) were higher than 0.999 indicating a
high level of linear relationship between the concentration of monochrotophos and peak area.
Index Terms- Reversed-Phase High Performance Liquid Chromatography (RP-HPLC), Pesticides Residue, Quechers
Approach, Correlation Coefficient.