Pre-Osteoblastic Cells Proliferative Property Of Bioactive Compounds In Germinated Brown Rice: A Promising In Vitro Potentials In Osteoporosis
Background: Application of brown rice in the area of biomedical research is increasing especially in the management of
chronic metabolic diseases, butadequate effort has not been made to study the cellular toxicity and proliferative ability of its
bioactives to properly ascertain its safety.
Method: In this study, different in vitro assays were carried out to determine cell proliferation using Trypan blue exclusion
assay (TBE) and Bromidine uracil assay(Brdu). Toxicity of the bioactives was measured by (MTT) and Neutral red assays
(N.R) on MC3T3-E1 osteoblastic cells at varying concentrations. Alkaline phosphatase activity(ALP), collagen and protein
synthesis of the cells were also quantified.
Results: A time-dependent, significant proliferative effect on the cells was observed at a concentration of 10μg/ml compared
to other concentrations of 100 and 1000μg/ml for TBE and Brdu (p<0.05). No toxicity was recorded at a concentration
between 0 and 1000μg/ml, using both MTT and N.R assays. An overall significant increase in alkaline phosphatase activity
(p=0.0194) was observed. Collagen and protein synthesis increases significantly in ASG treated cells (p<0.05). Significant
correlation was observed between Brdu and TBE results (r2= 0.842, p= 0.01).
Conclusions: GBR-bioactives stimulates bone cell proliferation in-vitro possibly by increasing ALP synthesis which leads to
new DNA formation, protein and collagen synthesis, and the bioactives has no toxic effect; further research is needed to
ascertain the possibility of using these compounds in the management of osteoporosis.
Keywords: Cellular toxicity, cell proliferation, GBR- Bioactives, Osteoporosis