Paper Title
Development And Validation Of Ondansetron Hydrochloride In Pharmaceutical Dosage Form By Rp-Hplc Method

A simple, rapid, economical, accurate, and precise reverse phase high performance liquid chromatography (RP-HPLC) method was developed and subsequently validated for the estimation of Ondansetron hydrochloride (ODH) in bulk and pharmaceutical dosage form. The analysis was carried out using Princeton SPHER C18 column (250 mm x 4.6 mm id, 5µ particle size) at 300 c temperature using mobile phase Buffer:Acetonitrile:Methanol (50:40:10% v/v/v) (pH 4.5 ± 0.05) at flow rate 1.0 ml/min. Quantification was achieved with UV detector at 248 nm. Retention time of Ondansetron hydrochloride was found to be 2.67 ± 0.05 min. Linearity was studied in the concentration range 10- 60 µg/ml for Ondansetron hydrochloride with a correlation coefficient of 0.9982. The proposed method was validated according to ICH guidelines with respect to linearity, accuracy, precision, robustness, LOD, and LOQ. The developed method with good symmetry, successfully applied for determination of Ondansetron hydrochloride in its pharmaceutical dosage form. Keywords: RP-HPLC, Ondansetron hydrochloride, Validation.