Heavy Metal Accumulation by Saccharomyces Cerevisiae Cells Expressing Cup1cystein-Rich Olygopeptides Targeted to The Inner Face of The Plasma Membrane
Cup1 is the Saccharomyces cervisiaemetallothionein responsible for buffering the surplus copper taken up by yeast cells. In this study we obtained S. cerevisiae cells armed with three metal-binding oligopeptides derived from Cup1 sequence targeted to the inner face of the plasma membrane. The three metal binding oligoppetidescorrespon to three cystein-rich metal-binding sequences (MeBSeq1-3) from Cup1 primary stucture. We describe the constructionof S. cerevisiaestrains expressing MeBSeq1-3 fused to the carboxy-terminus of amyristoylated green fluorescent protein (myrGFP). These strains were investigated in terms of tolerance to heavy metals exposure and heavy metal accumulation.
Keywords - Heavy Metal, Saccharomyces Cerevisiae, Cup1, Metal-Binding Sequence