Glioblastoma Produces Nitric Oxide (No) in Response to Activation Stimulus
Glioblastoma is the most common and lethal primary brain tumor in adults. In order to better understand the nature of the lesion, including the multiple roles of its most important signal molecule NO, a more exact tumor structure simulation in vitro is required. The aim of the presented research is to study the relationship between the level of endogenous NO and mitochondrial function in vitro at individual three-dimensional multicellular objectresolution. A close positive association has been revealed between endogenous NO production and mitochondrial membrane hyperpolarization that can support the higher intracellular level of energy required for cell activation, proliferation and movement. Keywords - Glioblastoma, 3D Spheroids In Vitro, Nitric Oxide (NO), Mitochondrial Membrane Potential (MMP), Image Analysis.