Importance of High Performance Thin Layer Chromatography and UV Spectroscopy in Analysis of Homoeopathic Drug Datura Ferox
High-performance Thin Layer Chromatography (HPTLC) and UV spectroscopy is an important tool for the
qualitative and quantitative phytochemical, chemo profiling and marker compound analysis of homoeopathic drugs/tinctures
(HT). According to the phytoequivalence concept the full HT can be seen as the active compound, because the several
constituents act together being responsible for its therapeutic effect. Datura ferox in Homoeopathy frequently used in the
treatment of bronchitis in asthma, mental illness, respiratory disorders, ADHD (attention deficit hyperactivity) and drug
detoxification. Present study reveals the LOD (4.09%), Total Ash (1.99%), Alcohol extractive values (5.24%), Water
extractive values (2.94%), Total solids (0.52%), Wt/ml (0.84g) and Alcohol content (72%). In UV Spectroscopy λmax.
observed at 209 & 325nm in HT. HPTLC analyses of in house HT were performed by using chloroform methanol (9:1, v/v)
as mobile phase. Under UV light (254nm), two spots appear at Rf 0.44, 0.53(brown). Under U.V (366nm), five spots appear
at Rf 0.36, 0.43, 0.51, 0.58, 0.81(blue). After spray with Anisaldehyde Sulphuric acid eight spots appears at Rf 0.36(light
orange), 0.43(orange), 0.57(blue), 0.62(Purple), 0.66(blue), 0.69(brown), 0.78(purple) and 0.83(blue). The present
physicochemical and phytochemical data are to be considered as Pharmacopoeial standards for aforesaid drug
Keywords - Datura ferox, Homoeopathic drug, Chemoprofiling, H.P.T.L.C fingerprint.