Differences on isoflavone Profiles According to Extraction Method in Lupin Seeds
Isoflavones are an extensively group of compounds that are used as dietary supplements due to their
phytoestrogenic activity. There are several methods for isoflavone measurement but all of them had been developed for soja
products. Thus, as part of our interest on bioprospecting native sources, whole seeds of Andean Lupin (Lupinus mutabilis)
grown in two places of Colombian Andes were assessed to measure their content of isoflavones. With the aim to identify the
most suitable method for routine isoflavone analysis, four methods of extraction were then compared in order to measure
total isoflavones through HPLC.
Key words - Isoflavones, HPLC, chemical profile, Andean lupine, aglycones, glucosides.