Paper Title
Antifungal Activity Test by Aqueous Solution of Ocimum Sanctum [Tulsi]

Abstract
In India many plants have religious value. Other than religious importance it has many medicinal properties. Since ancient time people used herbs as medicine because of its several uses and less side effects. Tulsi [Ocimum sanctum] have medical properties as well as antifungal and antimicrobial activity. Here Aspergillus niger was subjected to experiment. Leaf extraction of Ocimum was tested as antifungal agent. 25%, 50%, 75% and 100% of leaf extract concentration was tested on potato dextrose agar [PDA] for antifungal test at 27±1 ℃ for 3 days. Increasing in concentration of leaf extract inhibits the fungal mycelium growth. This study has been done by using aqueous extract of Ocimum sanctum Linn. leaf at different concentration i.e. 25%, 50%, 75%and 100% of solution made for the test. It was observed that 100% concentration of leaf extract was more effective in fungal growth inhibition. Plant extracts is easily found cheap and ecofriendly for control of fungal disease. Keywords - Ocimum sanctum, Fungal Mycelium, Aspergillus niger, Antifungal