Prospects for the use of Accelerated Methods and Portable Test Systems DNA and Immunodiagnostics for Monitoring Anthropogenic and Natural Toxicants in the Objects of Sanitary Surveillance under Non-Stationary Conditions
Conducted theoretical experimental assessment methods, portable test systems, DNA and immunoassay
(immunochromatographic indicatory elements, DNA and immunophenotype) for suitability in monitoring of natural and
anthropogenic toxicants in the objects of sanitary surveillance in non-stationary conditions.
Immunoassay analysis is based on the course of immune response "antigen-antibody" with the movement of the test liquid
sample according to a composite membrane and a visual accounting of the results by the presence or absence of painted
areas with immobilized immunoreagents. Evaluation of the results of immunoassay analysis were correlated with testimony
videocatalogue domain reflectometer "Relcom". The application of reflectometric registration increased sensitivity of
detection compared to visual observation result of the operator. However, reflectometry allows to reduce such factors of
subjectivity in the evaluation of the result of person as the illumination of the workplace, fatigue, or individual peculiarities
of perception of colors and saturation lines immunochromatography. Conducted experimental evaluation of methods for
determination of bacteria using DNA chips. The analysis was performed according to the modified method, including the
selection of bacteria, lysis and DNA clean-up.
Data analysis showed that hybridization occurs only in the homologous systems (immobilized DNA and a DNA probe of the
same species of bacteria.
Shown their high sensitivity, specificity, speed, and the possibility of implementing them in the field. Based on import
substitution, it is recommended to use a domestic analogy, which is not inferior in quality to foreign.
Keywords: Monitoring natural and anthropogenic toxicants, sanitary control facilities immunochromatographic display
elements (IDE) Immunomicrochip DNA Technology.