Paper Title
A Cross sectional Study on Antimicrobial Drug Resistance Pattern of Escherichia Coli Isolated from Humans Urine and Stool sample in Khulna

Escherichia coli (E.coli) is one of the most common causative organism for urinary tract infection (UTI) and diarrhoea in developing countries, specially in Bangladesh. We conducted a retrospective study of Escherichia coli isolates recovered from human urinary and stool samples during August, 2016–September, 2017 in Khulna city to generating primary data on the prevalence of antimicrobial drug resistance in E. coli in a sample population. In the present study a total of 524 E. coli isolates from human’s urinary sample and 437 from human stool sample, were tested for susceptibility to 12 antimicrobial drugs. Our result reveals that E.coli is 97-100% sensitive to imipenem and meropenem isolated from both urine and stool sample. Most extensively used antibiotic Ciprofloxacin has become 33% and 25% resistant isolated from urine and stool sample respectively. 98.85% and 98.90% resistance to ampicillin, 97.98% and 88.17% resistance to amikacin, 95.34% and 87.13% resistant to tetracycline, 83.7% and 91.37% resistance to erythromycin, 93.6% and 91.72% resistance to co-trimoxazol, 63.57% and 68.56% resistance to ceftriaxon, 89.16%and 92% resistance to trimethoprim-sulfomethoxazole, 98.2% and 96.3% resistance to, nalidaxic acid and 49.03% and 51.34% resistant to gentamycin (urine and stool sample respectively). These data indicate that the multidrug resistance of E. coli is gradually increasing in Khulna city. Although, population density is lower in Khulna and the study were perform in a short period of time. Further details study including genomic and proteomic basis of resistance pattern is needed. Index Terms - E. coli, Antimicrobial drug resistance, susceptibility, Multi drug resistance