Microbial Lipid Extraction by Electroporation Technique Culturing Lipomyces Starkeyi on Palm Oil Mill Effluent (POME)
Abstract - Microbial lipid is considered as a substitute of traditional edible oils like plant oil, animal fat, cooking oil etcetera
as feedstock of biodiesel. Oleaginous yeast strain Lipomyces starkeyi is a potential candidate can play a key role for
simultaneous bioremediation as well as lipid accumulation from hydrocarbon enriched palm oil mill effluent (POME). The
main obstacle to obtain higher lipid extraction from microbial biomass is the cell wall breakup. Therefore, the present study
is intended to develop a novel cell wall disruption technique for the extraction of biomolecules from microbial biomass
within a short time as well as eco-friendly. In this study, the electroporation (EP) technique was employed to disrupt yeast
cell where voltage, pulse frequency and pulse width were 4kV, 100 Hz and 0.01 s respectively. In addition, different
treatment time (2, 4, 8 min) were employed to observe the effect of EP treatment on cell inactivation as well as lipid
extraction. Our experimental results show that the maximum lipid extraction was achieved by employing 8 minutes EP
treatment. These results suggest that EP would be an attractive technique alternative to the traditional microbial cell
disruption that leads to significant lipid extraction.
Keywords - Palm oil mill effluent; Bioremediation;Lipomyces starkeyi; Electroporation; Microbial lipid.