ER-to-Plasma Membrane Tethering Proteins Regulate the Asymmetric Distribution of Pma1p During Cell Division in Saccharomyces cerevisiae
The endoplasmic reticulum (ER) makes a physical contact with various intracellular organelles, such as
mitochondria and lysosomes, as well as the plasma membrane (PM) via membrane contact sites. Although their cellular
functions remain largely unknown, the membrane contact site between ER and the PM is thought to mediate lipid exchange
between the two membranes. Here, we found that deletion of genes encoding ER-to-plasma membrane tethering proteins
markedly destroyed the asymmetric distribution of Pma1p, which regulates replicative lifespan in the budding yeast
Saccharomyces cerevisiae. Thus, our results suggest that ER-PM membrane contact sites regulate cell aging in yeast.
Index terms - Endoplasmic reticulum, Membrane contact site, Pma1p, Aging, Saccharomyces cerevisiae.