Paper Title :Antibiotic Resistance in Probiotic Lactic Acid Bacteria: A Safety Concern
Author :Ramachandra.B, Praveen. A.R, Rajunaik.B, Malashree.Lk, Prabha, R
Article Citation :Ramachandra.B ,Praveen. A.R ,Rajunaik.B ,Malashree.Lk ,Prabha ,R ,
(2017 ) " Antibiotic Resistance in Probiotic Lactic Acid Bacteria: A Safety Concern " ,
International Journal of Advances in Science, Engineering and Technology(IJASEAT) ,
pp. 33-38,
Volume-5, Issue-2, Spl. Iss-2
Abstract : Thirty domestic dahi samples collected from the different parts of north Bengaluru. 40 lactic isolates were
obtained from 30 domestic dahi samples. To determine the probiotic nature the lactic isolates were subjected for acid and
bile tolerance test and antibacterial compounds. Out of 40 lactic isolates only 21 lactic isolates showed varied tolerance to
acid and bile all the acid and bile tolerance and all the 13 lactobacilli isolates which were produced antibacterial compounds
against the indicator bacteria such as E.coli, Salmonella and Stap. aureusand other 9 isolates did not produced antibacterial
compounds. These 21 lactic isolateswere identified to species level phenotypically and further identity confirmed by 16S
rRNA sequencing. The identity revealed that 2 lactococci isolates as L.lactis ssp. lactis and L.lactis ssp. lactisbvdiacetylactis
13 lactobacilli isolates as L.rhamnosus(5 nos), L.plantarum (3 nos), L.fermnetum (3 nos), Lab.delbreukii ssp. bulgaricus (2
No.s) Leuconostocmesentroides ssp. mesentroides (3 nos) and S.thermophilus (3 nos). Further, both acid and bile and
antibacterial activity lactobacilli isolates selected for antibiotic screening. The lactic cultures do carry antibiotic genes, if the
antibiotic genes are transferable is a safety issue. Hence, in this study a screening test was conducted to determine whether
the isolates obtained are resistance or susceptible to the used antibitics and lesser the inhibitory zone more the resistance and
more the inhibitory zone indicates their susceptible nature. All the lactobacilli cultures used in this study were screened for
their antibiotic resistance or susceptibility against the seven commonly used antibiotics such as vancomycin,penicillin,
gentamycin, tetracycline, bacitracin, streptomycin, kanamycin. All the 5 strains of L.rhamnosus strains were susceptible to
all the 7 antibiotics tested as they showed more than 10 mm diameter inhibitory zone. Similarly, all the 3 strains
ofL.plantarum, all the 3 strains of L.fermentum and 2 strains of L.delbruckiissp. bulgaricus were susceptible to all the
antibiotics to varying degrees. These results clearly demonstrate the susceptible nature of the probiotic lactic isolates in this
study towards commonly used antibiotics.
Key words- Probiotic, Antibiotic Resistance, Lactic Acid Bacteria
Type : Research paper
Published : Volume-5, Issue-2, Spl. Iss-2
DOIONLINE NO - IJASEAT-IRAJ-DOIONLINE-8485
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Published on 2017-08-30 |
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